Journal article

Partitioning the roles of CYP6G1 and gut microbes in the metabolism of the insecticide imidacloprid in Drosophila melanogaster

Roberto Fusetto, Shane Denecke, Trent Perry, Richard AJ O'Hair, Philip Batterham



Resistance to insecticides through enhanced metabolism is a worldwide problem. The Cyp6g1 gene of the vinegar fly, Drosophila melanogaster, is a paradigm for the study of metabolic resistance. Constitutive overexpression of this gene confers resistance to several classes of insecticides, including the neonicotinoid imidacloprid (IMI). The metabolism of IMI in this species has been previously shown to yield oxidative and nitro-reduced metabolites. While levels of the oxidative metabolites are correlated with CYP6G1 expression, nitro-reduced metabolites are not, raising the question of how these metabolites are produced. Some IMI metabolites are known to be toxic, making their fate within the ..

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Funding Acknowledgements

The authors thank the University of Melbourne for the provision of the postgraduate and writing up scholarships to Roberto Fusetto and Shane Denecke and to all members of the O'Hair and Batterham research groups for their support. We thank Metabolomics Australia and the University of Melbourne Department of Chemistry for access to facilities and Sioe See Volaric for technical assistance with the analytical instrumentation.