Characterization of an osteoblast-like clonal cell line which responds to both parathyroid hormone and calcitonin.
SM Forrest, KW Ng, DM Findlay, VP Michelangeli, SA Livesey, NC Partridge, JD Zajac, TJ Martin
Calcif Tissue Int | Published : 1985
The clonal cell line UMR 106, which was originally derived from a rat transplantable osteogenic sarcoma with an osteoblastic phenotype, was subcloned after the emergence of a calcitonin-responsive adenylate cyclase was noted in late passages. Detailed studies on the stimulation of adenylate cyclase and activation profile of the cyclic AMP-dependent protein kinase isoenzymes in response to parathyroid hormone (PTH) and salmon calcitonin (SCT) were conducted on two subclones (UMR 106-01 and UMR 106-06). Both subclones responded in an identical manner to PTH, which stimulated adenylate cyclase and activated both isoenzyme I and isoenzyme II of cyclic AMP-dependent protein kinase. In contrast, o..View full abstract