Journal article

Transendothelial migration of lymphocytes in chronic lymphocytic leukaemia is impaired and involves down-regulation of both L-selectin and CD23

JR Chen, BJ Gu, LP Dao, CJ Bradley, SP Mulligan, JS Wiley

British Journal of Haematology | WILEY | Published : 1999

Abstract

Chronic lymphocytic leukaemia (B-CLL) is characterized by a progressive accumulation of B lymphocytes in blood and bone marrow and high concentrations of soluble CD23 and L-selectin are found in the serum of these patients. In this study lymphocytes from normal subjects and patients with B-CLL were allowed to undergo transendothelial migration across confluent layers of human umbilical vein endothelial cells. Lymphocytes in B-CLL samples showed an impaired capacity to migrate while the minor proportion of normal T cells was enriched by a mean of 2.5-fold in the transmigrated lymphocytes. In contrast, the ratio of B to T lymphocytes in normal preparations was unchanged in the transmigrated po..

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