Inhibition of cation cotransport by cholesterol enrichment of human red cell membranes.
JS Wiley, RA Cooper
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism | Published : 1975
1. Human red cells were enriched with cholesterol by incubation with lipid dispersions having a high cholesterol: phospholipid mol ratio and the kinetics of the furosemide-sensitive cotransport for Na+ and K+ were measured. 2. Influxes of both K+ and Na+ through this system were inhibited by 70 and 76% in cholesterol-rich cells (cholesterol: phospholipid mol ratio 1.80) and the Km of the furosemide-sensitive flux components for both K+ and Na+ decreased. 3. Effluxes of both K+ and Na+ are inhibited by furosemide and the magnitudes of these furosemide-sensitive components are markedly decreased in cholesterol-rich cells. 4. The inhibitory effect of cholesterol enrichment on this carrier-media..View full abstract