[Study on apoptosis of leukemic lymphocytes mediated by purinergic P2z receptors].
L Peng, CJ Bradley, JS Wiley
Chinese Journal of Hematology | Published : 1998
OBJECTIVE: To investigate the role of purinergic P2z receptors in human leukemic lymphocyte apoptosis induced by extracellular adenosine triphosphate (ATP). METHODS: Eleven B-CLL patients were studied. Leukemic lymphocytes with (n = 8) or without (n = 3) P2z receptors were exposed in vitro to ATP, benzoyl-benzoic-ATP (BzATP), 2-methylthio-ATP(2MeSATP), adenosine-5'-[gamma-thio] triphosphate (ATP-gamma S), and other nucleosides for 8 h. Apoptosis was detected by electron microscopy (EM), agarose gel electrophoresis, and quantitative TdT assay. RESULTS: Apoptosis was detected only in leukemic lymphocytes with P2z receptors. By using the quantitative assay, ATP-inducing DNA strand breaks were f..View full abstract