Cryopreservation of aoudad (Ammotragus lervia sahariensis) sperm obtained by transrectal ultrasound-guided massage of the accessory sex glands and electroejaculation
J Santiago-Moreno, C Castano, A Toledano-Diaz, MC Esteso, A Lopez-Sebastian, R Guerra, MJ Ruiz, N Mendoza, C Luna, JA Cebrian-Perez, TB Hildebrandt
THERIOGENOLOGY | ELSEVIER SCIENCE INC | Published : 2013
This study examines (1) the effectiveness of transrectal, ultrasound-guided massage of the accessory sex glands (TUMASG) combined with electroejaculation for obtaining aoudad (Ammotragus lervia sahariensis) sperm samples for cryopreservation, and (2) the effectiveness of a Tris-citric acid-glucose-based medium (TCG; usually used for freezing ibex sperm) and a TES-Tris-glucose-based medium (TTG; typically used in the cryopreservation of mouflon sperm) as sperm extenders. After TUMASG, just one to three electrical pulses were required for ejaculation to occur in five of the six animals studied; one ejaculated after TUMASG alone. Transrectal, ultrasound-guided massage of the accessory sex gland..View full abstract
Awarded by MCINN
This research was funded by MCINN grant AGL2011-25495. The authors thank the Parque Zoologico del Ayuntamiento de Cordoba for its unfailing help in the execution of this project.