Journal article

Germany/Australia Index of Sperm Sex Sortability in Elephants and Rhinoceros

B Behr, D Rath, TB Hildebrandt, F Goeritz, S Blottner, TJ Portas, BR Bryant, B Sieg, A Knieriem, SP de Graaf, WMC Maxwell, R Hermes



Flow cytometric sexing of spermatozoa followed by application in artificial insemination or in vitro fertilization provides a unique opportunity to predetermine the sex of offspring and might enhance the conservation management of endangered species in captivity such as the elephant and rhinoceros. To obtain an indication of the sortability of spermatozoa from these species, the relative DNA differences between X and Y chromosome bearing spermatozoa (fresh, frozen thawed, epididymal) from three rhinoceros species [white (Ceratotherium simum), black (Diceros bicornis), Indian (Rhinoceros unicornis)] and both elephant species, the Asian and the African elephant (Elephas maximus, Loxodonta Afri..

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University of Melbourne Researchers


Funding Acknowledgements

The authors are grateful to Antje Frenzel, Monica Ruckholdt and Christina Struckmann for assistance with the flow cytometer and Stephanie Holler for technical assistance. Animal care and animal training staff at Western Plains Zoo, Dubbo, NSW and Zoo Hannover, Hannover, Germany, are thanked for assistance with animal handling, in particular, Jurgen Kruse. This project was funded by the German Research Foundation (DFG), the German Academic Exchange Service (DAAD) and the Boehringer Ingelheim Fonds.