Journal article

Immunogenicity of a human immunodeficiency virus (HIV) polytope vaccine containing multiple HLA A2 HIVCD8( ) cytotoxic T-cell epitopes

T Woodberry, J Gardner, L Mateo, D Eisen, J Medveczky, IA Ramshaw, SA Thomson, RA French, SL Elliot, H Firat, FA Lemonnier, A Suhrbier

Journal of Virology | AMER SOC MICROBIOLOGY | Published : 1999

Abstract

Compelling evidence now suggests that alphabeta CD8 cytotoxic T lymphocytes (CTL) have an important role in preventing human immunodeficiency virus (HIV) infection and/or slowing progression to AIDS. Here, we describe an HIV type 1 CTL polyepitope, or polytope, vaccine comprising seven contiguous minimal HLA A2-restricted CD8 CTL epitopes conjoined in a single artificial construct. Epitope-specific CTL lines derived from HIV-infected individuals were able to recognize every epitope within the construct, and HLA A2-transgenic mice immunized with a recombinant virus vaccine coding for the HIV polytope also generated CTL specific for different epitopes. Each epitope in the polytope construct wa..

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