The Effects of Influenza A Virus PB1-F2 Protein on Polymerase Activity Are Strain Specific and Do Not Impact Pathogenesis
Julie L McAuley, Kelly Zhang, Jonathan A McCullers
Journal of Virology | AMER SOC MICROBIOLOGY | Published : 2010
The influenza A virus PB1-F2 protein has been implicated as a virulence factor, but the mechanism by which it enhances pathogenicity is not understood. The PB1 gene segment of the H1N1 swine-origin influenza virus pandemic strain codes for a truncated PB1-F2 protein which terminates after 11 amino acids but could acquire the full-length form by mutation or reassortment. It is therefore important to understand the function and impact of this protein. We systematically assessed the effect that PB1-F2 expression has on viral polymerase activity, accumulation and localization of PB1, and replication in vitro and in mice. We used both the laboratory strain PR8 and a set of viruses engineered to s..View full abstract
Awarded by PHS
Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
The work described here was supported by PHS grant AI-66349 and by ALSAC.We thank Samuel Connell and Jennifer Peters of the Cellular Imaging Shared Resource for technical assistance with confocal microscopy and helpful discussions about the data.