Journal article

INDUCTION OF CELL-SURFACE PEPTIDASE ACTIVITY - A GLOBAL RESPONSE TO CELL STRESS CORRELATED WITH APOPTOSIS

SB BROWN, RM KLUCK, KAO ELLEM

JOURNAL OF CELLULAR BIOCHEMISTRY | WILEY | Published : 1994

Abstract

We have previously characterized the stimulation of HeLa cell surface peptidase activity directed toward a nonapeptide substrate in response to low fluences of ultraviolet irradiation [Brown et al. (1993): J Cell Biochem 51:102-115]. To explore the hypothesis that this comprised a global response to cell stress featuring the interruption of DNA synthesis, a variety of agents affecting macromolecular synthesis were applied to HeLa cell cultures. Actinomycin D, 5,6-dichloro-1 beta-ribofuranosyl benzimadazole, mitomycin C, ultraviolet light, and cycloheximide at doses which inhibited cell growth, but fell short of increasing the proportion of cells which had lost cell membrane impermeability to..

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University of Melbourne Researchers