Journal article

cGAL, a temperature-robust GAL4-UAS system for Caenorhabditis elegans.

Han Wang, Jonathan Liu, Shahla Gharib, Cynthia M Chai, Erich M Schwarz, Navin Pokala, Paul W Sternberg

Nature Methods | Published : 2017

Abstract

The GAL4-UAS system is a powerful tool for manipulating gene expression, but its application in Caenorhabditis elegans has not been described. Here we systematically optimize the system's three main components to develop a temperature-optimized GAL4-UAS system (cGAL) that robustly controls gene expression in C. elegans from 15 to 25 °C. We demonstrate this system's utility in transcriptional reporter analysis, site-of-action experiments and exogenous transgene expression; and we provide a basic driver and effector toolkit.

University of Melbourne Researchers