Characterization of field isolates of Newcastle disease virus using antipeptide antibodies.
AN Hodder, ZY Liu, PW Selleck, GL Corino, BJ Shiell, DC Grix, CJ Morrow, JJ Gorman
Avian Dis | Published : 1994
A recent Australian field isolate of Newcastle disease virus (NDV) was analyzed with antipeptide antibodies capable of differentiating between the sequences at the cleavage activation sites of fusion proteins of different NDV pathotypes. The isolate was found to have the same fusion protein cleavage activation signal as the V4 isolate of the Queensland strain of NDV. However, the isolate failed to react with an antibody specific for the carboxyl-terminal extension on the hemagglutinin/neuraminidase (HN)-protein precursor (HNo-protein) of the V4 isolate and other similar strains (e.g., Ulster and D26). Identity of the fusion protein cleavage activation motif of the isolate was confirmed by ch..View full abstract