Identification of a new genetic marker in Mycoplasma synoviae vaccine strain MS-H and development of a strategy using polymerase chain reaction and high-resolution melting curve analysis for differentiating MS-H from field strains
Ling Zhu, Barbara M Konsak, Olusola M Olaogun, Rebecca Agnew-Crumptona, Anna Kanci, Marc S Marenda, Glenn F Browning, Amir H Noormohammadi
VETERINARY MICROBIOLOGY | ELSEVIER SCIENCE BV | Published : 2017
Mycoplasma synoviae (MS) is an economically important avian pathogen worldwide, causing subclinical respiratory tract infection and infectious synovitis in chickens and turkeys. A temperature-sensitive (ts+) live attenuated vaccine MS-H, derived from the Australian field strain 86079/7NS, is now widely used in many countries to control the disease induced by MS. Differentiation of MS-H vaccine from field strains is crucial for monitoring vaccination programs in commercial poultry. Comparison of genomic sequences of MS-H and its parent strain revealed an adenine deletion at nucleotide position 468 of the MS-H oppF-1 gene. This mutation was shown to be unique to MS-H in further comparative ana..View full abstract
The authors would like to acknowledge the assistance from the staff of the Asia-Pacific Centre for Animal Health (APCAH), Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne. Funding for this study was provided by APCAH.