A T cell-specific knockout reveals an important role for protease-activated receptor 2 in lymphocyte development
Nidhish Francis, Alison L Every, Babatunde A Ayodele, Robert N Pike, Eleanor J Mackie, Charles N Pagel
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2017
Activation of protease-activated receptor-2 (PAR2) expressed by T cells has been linked to the bone loss associated with periodontitis. We generated PAR2 conditional-null mice and crossed these with mice expressing Cre recombinase under control of the Lck proximal promoter, to produce T cell-specific PAR2-null mice in order to further study the cellular mechanism involved in periodontitis. Here we report that efficient deletion of PAR2 in thymocytes isolated from T cell-specific PAR2-null mice resulted in thymic and splenic hypoplasia and a reduction in the cells of the cortex and a loss of distinction between the cortex and the medulla of the thymus. FACS analysis confirmed significant redu..View full abstract
Awarded by National Health and Medical Research Council
This work was supported by the National Health and Medical Research Council [project grant number 1044103].