Journal article
Deoxyribonuclease 1 reduces pathogenic effects of cigarette smoke exposure in the lung
PT King, R Sharma, KM O'Sullivan, J Callaghan, L Dousha, B Thomas, S Ruwanpura, S Lim, MW Farmer, BR Jennings, M Finsterbusch, G Brooks, S Selemidis, GP Anderson, SR Holdsworth, PG Bardin
Scientific Reports | NATURE PORTFOLIO | Published : 2017
Abstract
Our aim was to investigate if deoxyribonuclease (DNase) 1 is a potential therapeutic agent to reduce pathogenic effects of cigarette smoke exposure in the lung. Cigarette smoke causes protease imbalance with excess production of proteases, which is a key process in the pathogenesis of emphysema. The mechanisms responsible for this effect are not well-defined. Our studies demonstrate both in vitro and in vivo that cigarette smoke significantly increases the expression of neutrophil and macrophage extracellular traps with coexpression of the pathogenic proteases, neutrophil elastase and matrix metalloproteinases 9 and 12. This response to cigarette smoke was significantly reduced by the additi..
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Funding Acknowledgements
The authors would like to thank the staff of Clinical Immunology at Monash Health, Alex Fulcher, Kirstin Elgass and Camden Lo of Monash Micro Imaging (MMI) for help with the taking of the confocal microscopy pictures and the authors acknowledge the MMI facilities of Monash University. The authors acknowledge the facilities and scientific and technical assistance of the Monash Histology Platform. This study was supported by a Monash University Strategic Grant and the Monash Lung and Sleep Institute.