THE BETA-SUBUNIT OF THE GASTRIC H /K -ATPASE CAN OCCUR WITHOUT THE ALPHA-SUBUNIT

Abstract

The renal Na+/K(+)-ATPase and the gastric H+/K(+)-ATPase both contain alpha- and beta-subunits. We report here the identification and partial purification of a second population of the beta-subunit of the gastric H+/K(+)-ATPase, which has no accompanying alpha-subunit detectable by Coomassie blue staining or by Western blotting with monoclonal antibodies specific for the alpha-subunit.