PLATELET-DERIVED GROWTH-FACTOR INDUCES PHOSPHATIDYLINOSITOL 3-KINASE RELEASE FROM THE MIDDLE T-PP60(C-SRC) COMPLEX AND ASSOCIATION WITH THE PLATELET-DERIVED GROWTH-FACTOR RECEPTOR

Abstract

Both platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) induce mitogenesis in normal rat kidney (NRK) fibroblasts transformed by the polyoma virus middle T (pmt) oncogene. In unstimulated pmt-NRK cells phosphatidylinositol 3-kinase forms a complex with the middle T protein and pp60c-src. PDGF treatment causes a release of phosphatidylinositol 3-kinase activity from the complex and a simultaneous increase in activity associated with the PDGF receptor. In contrast after treatment with EGF the majority of phosphatidylinositol 3-kinase activity remains associated with the middle T-pp60c-src complex. Proliferation of NRK fibroblasts transformed by the v-src oncogene is alread..