Journal article

Quantitative measurement of mRNA coding for the receptors controlling acid secretion in the ovine fundus and antrum by using RT-PCR

S Kolivas, R Dow, R Jie, GS Baldwin, A Shulkes

JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY | BLACKWELL SCIENCE ASIA | Published : 2000

Abstract

Background and Aims: Gastric acid secretion is stimulated by the action of gastrin, histamine and acetylcholine on their respective receptors. To determine the regulation of synthesis of these receptors during different gastric secretory states a competitive RT-PCR method for quantitating the mRNA for these receptors was developed. Methods: Partial cDNA clones (400-500 base pairs (bp)) for the ovine gastrin, histamine (H2) and acetylcholine (M3) receptors were isolated and sequenced. These cDNA constructs were modified by the inclusion of approximately 100 bp of unrelated sequence within the plasmids. cDNA was synthesized from a mixture of known amounts of RNA transcribed from the modified p..

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University of Melbourne Researchers