Plasmodium falciparum complementary DNA clones expressed in Escherichia coli encode many distinct antigens.
RF Anders, RL Coppel, GV Brown, RB Saint, AF Cowman, KR Lingelbach, GF Mitchell, DJ Kemp
Mol Biol Med | Published : 1984
A library of cDNA clones expressing antigens of the asexual blood-stages of Plasmodium falciparum (isolate FCQ27/PNG) was constructed in the bacteriophage vector gamma gt11-Amp3. Clones expressing P. falciparum antigens (as polypeptides fused to beta-galactosidase) were selected by their reactivity in an in situ colony immunoassay with affinity-purified malaria antibodies. A detailed analysis of 78 antigen-positive clones selected from approximately 10,000 recombinant clones has shown them to correspond to many different parasite antigens. cDNA hybridization studies on this array of 78 antigen-positive clones have so far identified 18 families of sibling clones with 22 clones as yet unassign..View full abstract