Journal article
The expression of Plasmodium falciparum bloodstage antigens in Escherichia coli.
GV Brown, RF Anders, RL Coppel, RB Saint, AF Cowman, HD Stahl, KR Lingelbach, GF Mitchell, MP Alpers, DJ Kemp
Philosophical Transactions of the Royal Society of London Series B Biological Sciences | ROYAL SOC | Published : 1984
Abstract
A library of cDNA clones expressing proteins of the asexual blood stages of a Papua New Guinean isolate of Plasmodium falciparum (isolate FCQ27/PNG (FC27] was constructed in the bacteriophage vector lambda gt11-Amp3. In an in situ colony immunoassay, human serum was used to identify colonies producing natural immunogens. Sera from donors of defined clinical status, or reactive to a defined subset of natural immunogens were used to identify clones of particular interest (for example, clones reacting with convalescent but not with acute serum or clones expressing the isolate specific S-antigen of FC27). Antisera raised by immunizing mice and rabbits with cloned antigens were used to characteri..
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