Journal article

Pivotal Advance: Peritoneal cavity B-1 B cells have phagocytic and microbicidal capacities and present phagocytosed antigen to CD4( ) T cells

David Parra, Aja M Rieger, Jun Li, Yong-An Zhang, Louise M Randall, Christopher A Hunter, Daniel R Barreda, J Oriol Sunyer

JOURNAL OF LEUKOCYTE BIOLOGY | FEDERATION AMER SOC EXP BIOL | Published : 2012

Abstract

Breaking the long-held paradigm that primary B cells are not phagocytic, several studies have demonstrated recently that B cells from fish, amphibians, and reptilians have a significant phagocytic capacity. Whether such capacity has remained conserved in certain mammalian B cell subsets is presently an enigma. Here, we report a previously unrecognized ability of PerC B-1a and B-1b lymphocytes to phagocytose latex beads and bacteria. In contrast, B-2 lymphocytes had an almost negligible ability to internalize these particles. Upon phagocytosis, B-1a and B-1b cells were able to mature their phagosomes into phagolysosomes and displayed the ability to kill internalized bacteria. Importantly, B-1..

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Funding Acknowledgements

This work was supported by a 2008 University of Pennsylvania-School of Veterinary Medicine VCID award to J.O.S., a 2010 University of Pennsylvania Research Foundation (URF) award to J.O.S., and a NIH grant to J.O.S. (R01GM085207-01). This study was partially supported by a Natural Sciences and Engineering Council of Canada (NSERC) research grant to D.R.B. We thank Jeffrey S. Faust and the staff of the Flow Cytometry Facility (The Wistar Institute) for the cell-sorting procedures. We also thank Y. Liu (Penn Vet Imaging Facility, University of Pennsylvania) and R. Meade (Biomedical Imaging Core, School of Medicine, University of Pennsylvania).