Safety and efficacy of a Mycoplasma gallisepticum oppD knockout mutant as a vaccine candidate
Chi-Wen Tseng, Chien-Ju Chiu, Anna Kanci, Amir H Noormohammadi, Glenn F Browning, Philip F Markham
VACCINE | ELSEVIER SCI LTD | Published : 2017
Control of the important poultry pathogen Mycoplasma gallisepticum is highly dependent on safe and efficacious attenuated vaccines. In order to assess a novel vaccine candidate we evaluated the safety and efficacy of the M. gallisepticum mutant 26-1. The oppD1 gene in this mutant has been interrupted by a signature-tagged transposon and previous studies have shown that it can colonise the respiratory tract of chickens without inducing significant disease. The capacity of the oppD1 mutant to induce protective immunity in the respiratory tract after vaccination by eye-drop was assessed by challenging vaccinated birds with an aerosol of the virulent M. gallisepticum strain Ap3AS. Vaccination wi..View full abstract
This work was partially supported by funding from the Australian Egg Corporation Ltd and the Australian Poultry CRC. We would like to thank Ms Cheryl Colson and Mr Tony Belfiore for their assistance with these studies.