Conference Proceedings

Identification of imidazoline-receptor binding sites in cortex and medulla of the bovine adrenal gland - Colocalization with MAO-A and MAO-B

PR King, AL Gundlach, WJ Louis, M Gothert (ed.), GJ Molerings (ed.), DJ Reis (ed.)

IMIDAZOLINE RECEPTORS AND THEIR ENDOGENOUS LIGANDS | NEW YORK ACAD SCIENCES | Published : 1999

Abstract

The distribution and relative densities of imidazoline-receptor binding sites (I-RBS) in bovine adrenal gland were determined using [3H]clonidine, [3H]2-(2-benzofuranyl)-2-imidazoline ([3H]2-BFI), and [3H]rilmenidine. In light of strong evidence that I-RBS and monoamine amine oxidase enzymes are linked, the selective radioligands [3H]RO41-1049 and [3H]RO19-6327 were used to label the distribution of MAO-A and -B enzymes, respectively. [3H]Clonidine (12 nM) labeled sites in two discrete regions of the bovine adrenal gland, the zona glomerulosa (39 +/- 7 fmol/mg tissue equivalent) and inner medulla (34 +/- 1 fmol/mg tissue). Binding was nonadrenergic (i.e., not inhibited by 100 nM methoxyidazo..

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