Journal article

AUTORADIOGRAPHIC LOCALIZATION OF SIGMA-RECEPTOR BINDING-SITES IN GUINEA-PIG AND RAT CENTRAL-NERVOUS-SYSTEM WITH ( )H-3-3-(3-HYDROXYPHENYL)-N-(1-PROPYL)PIPERIDINE

AL GUNDLACH, BL LARGENT, SH SNYDER

JOURNAL OF NEUROSCIENCE | SOC NEUROSCIENCE | Published : 1986

Abstract

(+)3H-3-PPP [(+)3H-3-(3-Hydroxyphenyl)-N-(1-propyl)-piperidine] binds with high affinity to brain membranes with a pharmacological profile consistent with that of sigma receptors. The distribution of (+)3H-3-PPP binding sites in brain and spinal cord of both guinea pig and rat has been determined by in vitro autoradiography with binding densities quantitated by computer-assisted densitometry. (+)3H-3-PPP binding to slide-mounted brain sections is saturable and displays high affinity and a pharmacological specificity very similar to sites labeled in homogenates. (+)3H-3-PPP binding sites are heterogeneously distributed. Highest concentrations of binding sites occur in spinal cord, particularl..

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University of Melbourne Researchers