Journal article

SPECIFIC BINDING OF MURINE LEUKEMIA INHIBITORY FACTOR TO NORMAL AND LEUKEMIC MONOCYTIC CELLS

DJ HILTON, NA NICOLA, D METCALF

Proceedings of the National Academy of Sciences of the United States of America | NATL ACAD PRESS | Published : 1988

Abstract

Leukemia inhibitory factor (LIF), a glycoprotein capable of suppressing the clonogenicity and inducing the differentiation of the murine myeloid leukemia cell line M1, was radioiodinated to a high specific radioactivity with retention of full biological activity. Binding of 125I-labeled LIF to M1 cells reached a steady state at 37 degrees C after approximately equal to 40 min and was in competition with unlabeled LIF but not granulocyte colony-stimulating factor or a range of other cytokines or differentiation-inducing agents. Specific binding was demonstrable to cells from a range of murine hemopoietic tissues including the bone marrow, the spleen, and the peritoneal cavity. Autoradiography..

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