Specific binding of murine leukemia inhibitory factor to normal and leukemic monocytic cells

Abstract

Leukemia inhibitory factor (LIF), a glycoprotein capable of suppressing the clonogenicity and inducing the differentiation of the murine myeloid leukemia cell line M1, was radioiodinated to a high specific radioactivity with retention of full biological activity. Binding of 125I-labeled LIF to M1 cells reached a steady state at 37°C after ~40 min and was in competition with unlabeled LIF but not granulocyte colony-stimulating factor or a range of other cytokines or differentiation-inducing agents. Specific binding was demonstrable to cells from a range of murein hemopoietic tissues including the bone marrow, the spleen, and the peritoneal cavity. Autoradiography, revealed macrophages, monocy..