Journal article

STRUCTURAL CHARACTERIZATION OF A MURINE MYELOID-LEUKEMIA INHIBITORY FACTOR

RJ SIMPSON, DJ HILTON, EC NICE, MR RUBIRA, D METCALF, DP GEARING, NM GOUGH, NA NICOLA

EUROPEAN JOURNAL OF BIOCHEMISTRY | SPRINGER VERLAG | Published : 1988

Abstract

A leukaemia inhibitory factor (LIF) which induces macrophage differentiation in M1 murine myeloid leukaemia cells and suppresses their proliferation in vitro has been isolated in sufficient quantities (30 micrograms) from Krebs ascites tumour cell conditioned medium to permit its partial characterization by amino acid sequence analysis. The combination of sensitive microbore column (1.0 and 2.1 mm internal diameter) HPLC technology and microsequence analysis has enabled the positive identification of 125 of the total 179 amino acid residues (70%) in the molecule. The amino acid sequence data reported here permitted the isolation of a partial cDNA clone encoding LIF [Gearing et al. (1987) EMB..

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