Inhibition of Differentiation in a Murine F9 Embryonal Carcinoma Cell Subline by Leukemia Inhibitory Factor (LIF)
GS Brown, MA Brown, D Hilton, NM Gough, MJ Sleigh
Growth Factors | TAYLOR & FRANCIS LTD | Published : 1992
Leukemia inhibitory factor (LIF) is a cytokine previously shown to maintain pluripotent embryonic stem cells in their undifferentiated state. We have examined the effects of LIF in nullipotent embryonal carcinoma cell lines, and have found that LIF blocks differentiation induced by retinoic acid and at low temperature in OTF9 cells. LIF did not block differentiation in a parent F9 cell line. For OTF9 cells, LIF acts early in differentiation, inhibiting the appearance of parietal endoderm-type product cells. However, it acts subsequent to retinoic acid, and at least one early retinoic acid-induced event is unaltered in the presence of LIF. This finding provides both a means of dissecting the ..View full abstract
The authors are grateful to Lindsay Williams for advice in the early stages of the project, to Angeles Sanchez and Trevor Lockett for critical reading of the manuscript and to Gerry Both for provision of oligonucleotides. The work in the laboratory of M. J. Sleigh was supported in part by a grant from the Australian Meat and Livestock Research and Development Corporation. Work at the Walter and Eliza Hall Institute was supported by the Anti-Cancer Council of Victoria, the National Health and Medical Research Council and AMRAD Corporation.