Cellular Processing of Murine Colony-Stimulating Factor (Multi-CSF, GM-CSF, G-CSF) Receptors by Normal Hemopoietic Cells and Cell Lines
Nicos A Nicola, Linda Peterson, Douglas J Hilton, Donald Metcalf
Growth Factors | TAYLOR & FRANCIS LTD | Published : 1988
The binding, internalization and degradation rates of three different murine colony-stimulating factors (Multi-CSF or interleukin-3, GM-CSF and G-CSF) and their receptor turnover rates were determined for normal bone marrow cells and a number of different cell lines at 37 degrees C. The kinetic parameters were extracted from a curve-fitting analysis of the approach to steady-state of surface-bound and internalized CSFs by methods described by Myers et al. (1987). The primary binding kinetic constants (association and dissociation) for each CSF on different cell types were similar, suggesting a single type of receptor for each CSF. In all cases, CSF binding induced a faster rate of internaliz..View full abstract
Awarded by National Institutes of Health, Bethesda, MD
Dr. T. M. Dexter (Paterson Laboratories, Manchester, U.K.), Dr. J. Ihle (Frederick Cancer Research Centre, Frederick, MD) and Dr. J. D. Watson (Auckland University, Auckland, N.Z.) are thanked for generously providing the FDCP-1, NFS-60 and 32D Cl.13 cells, respectively. Dr. J. DeLamarter (Biogen, Switzerland) is thanked for supplying recombinant murine GM-CSF and Multi-CSF and Dr. S. Vuk-Pavlovic (Mayo Foundation, Rochester) for providing us with the curve-fitting program. Drs. J. Coventry, A. Kyne and H. Davies are thanked for modifying the program for the VAXll computer. This work was supported by the National Health and Medical Research Council, Canberra, The Anti-Cancer Council of Victoria, the J. D. and L. Harris Trust Fund and the National Institutes of Health, Bethesda, MD, Grant CA-22556.