Conference Proceedings
Proteomic analysis of a response to long-chain fatty acid in Escherichia coli and its application
Mee-Jung Han, Jeong Wook Lee, Sang Yup Lee, Jong Shin Yoo, D Howard (ed.), PK Rhee (ed.), S Halgamuge (ed.), SJ Yoo (ed.)
PROCEEDINGS OF THE FRONTIERS IN THE CONVERGENCE OF BIOSCIENCE AND INFORMATION TECHNOLOGIES | IEEE COMPUTER SOC | Published : 2007
Abstract
A long-chain fatty acid is a regulatory signal that modulates gene expression. In this study, proteomic studies that compared global translational differences between Escherichia coli cells grown in the presence of exogenous glucose and oleic acid were examined by two-dimensional gel electrophoresis (2-DE) and tandem mass spectrometry (LC-MS/MS). Of about 40 proteins showing altered expression levels with the presence of oleic acid, 10 including AldA, AceA, FadA, FadB, FadL, MalE, RbsB, Udp, Cdd and YccU were newly synthesized. Among them, the promoter of aldehyde dehydrogenase A (aldA promoter) was used for the production of a recombinant protein. Analysis of fluorescence intensities and co..
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Awarded by Korean Systems Biology Research
Funding Acknowledgements
We thank Z. W. Lee (Korea Basic Science Institute, Daejeon, Republic of Korea) for his help with the confocal microscopy. This work was supported by the Korean Systems Biology Research Grant (M10309020000-03B5002- 00000) of the Ministry of Science and Technology. Further supports by LG Chem Chair Professorship, IBM SUR program, Microsoft, and by the KOSEF through the Center for Ultramicrochemical Process Systems are appreciated.