Studies on phytanoyl-CoA 2-hydroxylase and synthesis of phytanoyl-coenzyme A
NJ Kershaw, M Mukherji, CH MacKinnon, TDW Claridge, B Odell, AS Wierzbicki, MD Lloyd, CJ Schofield
Bioorganic & Medicinal Chemistry Letters | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2001
Phytanoyl-CoA 2-hydroxylase (PAHX), an iron(II) and 2-oxoglutarate-dependent oxygenase, catalyses an essential step in the mammalian metabolism of beta-methylated fatty acids. Phytanoyl-CoA was synthesised and used to develop in vitro assays for PAHX. The product of the reaction was confirmed as 2-hydroxyphytanoyl-CoA by NMR and mass spectrometric analyses. In accord with in vivo analyses, hydroxylation of both 3R and 3S epimers of the substrate was catalysed by PAHX. Both pro- and mature- forms of PAHX were fully active.