Localization of messenger ribonucleic acid for insulin-like growth factor-binding proteins in human skin by in situ hybridization.
JA Batch, FA Mercuri, SR Edmondson, GA Werther
J Clin Endocrinol Metab | Published : 1994
The role of the insulin-like growth factors (IGFs) in human skin physiology has been increasingly recognized, although relatively little is known about the cell types involved or the cellular mechanisms that mediate these responses. Epidermal keratinocytes and dermal fibroblasts both possess IGF-I receptors and are responsive to IGF-I. IGF-binding proteins (IGFBPs), known modulators of IGF action, may also be responsible for targeting IGF-I to its receptors and are produced by both cultured keratinocytes and fibroblasts. To demonstrate sites of production of IGFBPs in human skin, we have used in situ hybridization to localize messenger ribonucleic acid (mRNA) for the six IGFBPs. Antisense an..View full abstract