The innate immune receptor MDA5 limits rotavirus infection but promotes cell death and pancreaticinflammation
Yu Dou, Howard CH Yim, Carl D Kirkwood, Bryan RG Williams, Anthony J Sadler
The EMBO Journal | WILEY | Published : 2017
Awarded by National Health and Medical Research Council of Australia
We are grateful to Dr Marco Colonna for the Ifih1<SUP>-/-</SUP> mouse; Dr Daniel Cowley for purified RV; Dr Ganes Sen for anti-P56 antibody; Dr Paul Hertzog for Ifnar1<SUP>-/-</SUP> MEFs and Ifnar1-neutralizing antibody; Dr Nicole De Weerd for recombinant Ifn beta and Ifn1 alpha; Drs Curt Hovath, John Hiscott, and Ashley Mansell for expression constructs (MDA5, LGP2, MAVS, IRF3-5D, and dominant-negative IKK epsilon, IRF3, and TBK1); and Drs Kirstin Elgass and Sarah Creed for conducting the Cellomics analysis. We also thank Dr Aneta Strzelecki for technical support, Dr Frances Cribbin for editing this manuscript, and Drs Len Harrison and Margo Honeyman for their advice. This research was supported by grants from the National Health and Medical Research Council of Australia (Grantor ID: 491094 (BRGW) and 1043398 (AJS, BRGW); https://www.nhmrc.gov.au), a Monash University-China Scholarship Council Doctoral Scholarship (YD; http://www.monash.edu), and the Victorian Government's Operational Infrastructure Support Program (http://www.vic.gov.au). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.