Characterization of pancreatic islet cell infiltrates in NOD mice: effect of cell transfer and transgene expression.
LA O'Reilly, PR Hutchings, PR Crocker, E Simpson, T Lund, D Kioussis, F Takei, J Baird, A Cooke
Eur J Immunol | Published : 1991
Insulin-dependent diabetes mellitus can be transferred into young irradiated non-obese diabetic (NOD) mice by spleen cells from a diabetic NOD donor. T cells (both L3T4+ and Ly-2+) enter the pancreas 2 weeks following transfer. They are present initially at peri-islet locations but progressively infiltrate the islet with accompanying beta cell destruction. The infiltrate is heterogeneous with respect to V beta usage. Inflammatory macrophages (Mac-1+, F4/80+) can be detected at peri-islet locations at 1 week after transfer and continue to be recruited during the disease process. Their presence at the initiation of disease suggests that their primary function may be autoantigen presentation. I..View full abstract