Journal article

Disrupting assembly of the inner membrane complex blocks Plasmodium falciparum sexual stage development

Molly Parkyn Schneider, Boyin Liu, Philipp Glock, Annika Suttie, Emma McHugh, Dean Andrew, Steven Batinovic, Nicholas Williamson, Eric Hanssen, Paul McMillan, Marion Hliscs, Leann Tilley, Matthew WA Dixon

PLOS PATHOGENS | PUBLIC LIBRARY SCIENCE | Published : 2017

Abstract

Transmission of malaria parasites relies on the formation of a specialized blood form called the gametocyte. Gametocytes of the human pathogen, Plasmodium falciparum, adopt a crescent shape. Their dramatic morphogenesis is driven by the assembly of a network of microtubules and an underpinning inner membrane complex (IMC). Using super-resolution optical and electron microscopies we define the ultrastructure of the IMC at different stages of gametocyte development. We characterize two new proteins of the gametocyte IMC, called PhIL1 and PIP1. Genetic disruption of PhIL1 or PIP1 ablates elongation and prevents formation of transmission-ready mature gametocytes. The maturation defect is accompa..

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Grants

Awarded by Deutsche Forschungsgemeinschaft


Awarded by Australian Research Council


Awarded by National Health and Medical Research Council


Awarded by National Health and Medical Research Council Training fellowship


Funding Acknowledgements

MH was supported by the Deutsche Forschungsgemeinschaft (HL65/1-1) (http://www.dfg.de/en/index.jsp). LT is a Georgina Sweet, Australian Research Council Laureate Fellow (LE150100011) (http://www.arc.gov.au). MWAD and LT thank the Australian Research Council (DP110100624) (http://www.arc.gov.au) and the National Health and Medical Research Council (1098992) (https://www.nhmrc.gov.au) for funding this work. MWAD was supported by a National Health and Medical Research Council Training fellowship (602541). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.