Journal article
Differentiation of human embryonic stem cells and induced pluripotent stem cells to cardiomyocytes: A methods overview
CL Mummery, J Zhang, ES Ng, DA Elliott, AG Elefanty, TJ Kamp
Circulation Research | LIPPINCOTT WILLIAMS & WILKINS | Published : 2012
Abstract
Since human embryonic stem cells were first differentiated to beating cardiomyocytes a decade ago, interest in their potential applications has increased exponentially. This has been further enhanced over recent years by the discovery of methods to induce pluripotency in somatic cells, including those derived from patients with hereditary cardiac diseases. Human pluripotent stem cells have been among the most challenging cell types to grow stably in culture, but advances in reagent development now mean that most laboratories can expand both embryonic and induced pluripotent stem cells robustly using commercially available products. However, differentiation protocols have lagged behind and in..
View full abstractGrants
Awarded by National Institute of Biomedical Imaging and Bioengineering
Funding Acknowledgements
C.L.M. receives laboratory funding from the Netherlands Heart Foundation, EU FP7 ("InduStem" PIAP-GA-2008-230675), ZonMW (114000101), The Netherlands Institute of Regenerative Medicine, and the Netherlands Proteomics Consortium (050-040-250). A. G. E. is a Senior Research Fellow of the National Health and Medical Research Council (NHMRC) and receives laboratory funding from the NHMRC, the Australia Research Council, the Australian Stem Cell Centre, the Juvenile Diabetes Research Foundation, and the Queensland Neurosurgery Research Foundation (QNRF). D. A. E. is funded by the Australian Stem Cell Centre, NHRMC, and QNRF. T.J.K. receives funding from the National Institutes of Health (R01EB007534, R01HL08416, and U01HL099773).