The level of the tissue-specific factor GATA-1 affects the cell-cycle machinery.
DJ Whyatt, A Karis, IC Harkes, A Verkerk, N Gillemans, AG Elefanty, G Vairo, R Ploemacher, F Grosveld, S Philipsen
Genes Funct | Published : 1997
GATA-1 is a tissue-specific DNA-binding protein containing two zinc-finger-like domains. It is expressed predominantly in erythrocytes. Consensus binding sites for GATA-1 have been found in the regulatory elements of all erythroid-specific genes examined. GATA-1 protein is required for erythroid differentiation beyond the proerythroblast stage. In this paper, we demonstrate that the overexpression of GATA-1 in murine erythroleukaemia (MEL) cells alleviates DMSO-induced terminal erythroid differentiation. Hence, there is no induction of globin gene transcription and the cells do not arrest in the G1 phase of the cell cycle. Furthermore, we demonstrate that expression of GATA-1 in non-transfor..View full abstract