Journal article
Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming
X Liu, CM Nefzger, FJ Rossello, J Chen, AS Knaupp, J Firas, E Ford, J Pflueger, JM Paynter, HS Chy, CM O'Brien, C Huang, K Mishra, M Hodgson-Garms, N Jansz, SM Williams, ME Blewitt, SK Nilsson, RB Schittenhelm, AL Laslett Show all
Nature Methods | NATURE PUBLISHING GROUP | Published : 2017
DOI: 10.1038/nmeth.4436
Abstract
Recent reports on the characteristics of naive human pluripotent stem cells (hPSCs) obtained using independent methods differ. Naive hPSCs have been mainly derived by conversion from primed hPSCs or by direct derivation from human embryos rather than by somatic cell reprogramming. To provide an unbiased molecular and functional reference, we derived genetically matched naive hPSCs by direct reprogramming of fibroblasts and by primed-to-naive conversion using different naive conditions (NHSM, RSeT, 5iLAF and t2iLGöY). Our results show that hPSCs obtained in these different conditions display a spectrum of naive characteristics. Furthermore, our characterization identifies KLF4 as sufficient f..
View full abstractGrants
Awarded by Monash University
Funding Acknowledgements
We thank the high-quality cell sorting service and technical input provided by Monash Flowcore Facility and J. Hatwell-Humble for conducting the teratomas experiments. We also thank the Cytogenetics department of Monash Pathology for help with G-banding karyotype analysis. Furthermore, the authors thank the ACRF Centre for Cancer Genomic Medicine at the MHTP Medical Genomics Facility for assistance with next-generation library preparation and Illumina sequencing. This work was supported by National Health and Medical Research Council (NHMRC) project grants APP1104560 to J.M.P. and A.L.L., APP 1069830 to R.L. and a Monash University strategic grant awarded to C.M.N. X.L. was supported by a Monash International Postgraduate Research Scholarship and a Monash Graduate Scholarship. A.S.K. was supported by an NHMRC Early Career Fellowship APP1092280. J.M.P. and R.L. were supported by Silvia and Charles Viertel Senior Medical Research Fellowships.