Journal article

A SPIN LABEL STUDY OF THE IONIC-STRENGTH DEPENDENT CONFORMATIONAL CHANGE IN THE HUMAN IA MOLECULE

CC CURTAIN, R TOSI, FD LOONEY

MOLECULAR IMMUNOLOGY | PERGAMON-ELSEVIER SCIENCE LTD | Published : 1985

Abstract

Purified human Ia molecules were labelled with maleimide or isothiocyanate spin labels or by reacting "TEMPAMINE" spin label with the neuraminic acid of their carbohydrate residues. It was found that increasing the ionic strength from 0.05 to 0.75 markedly increased the dipolar interaction between the maleimide-attached labels, but no effect was found of ionic strength or motion on dipolar interaction with the other two labels. The effect of increasing ionic strength could be blocked by the prior addition of Ia-specific antibody, but could not be reversed by the addition of antibody after ionic strength was increased. These findings complement an earlier finding that increasing ionic strengt..

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University of Melbourne Researchers