Journal article

FATTY-ACID SPIN PROBE INTERACTIONS WITH ERYTHROCYTE-GHOSTS AND LIPOSOMES PREPARED FROM ERYTHROCYTE-GHOSTS

LM GORDON, FD LOONEY, CC CURTAIN

JOURNAL OF MEMBRANE BIOLOGY | SPRINGER | Published : 1989

Abstract

A model for the binding of 5-nitroxide stearate, I(12.3), to human erythrocyte ghosts was developed by comparing spin probe interactions with ghosts and liposomes prepared from ghosts. At low probe/lipid (P/L less than 1/2500), I(12.3) binds to a similar class of high-affinity, noninteracting sites in both ghosts and liposomes, indicating that lipid moieties are responsible for probe uptake. Saturation occurs in both systems with increasing P/L, and, at higher loading (e.g., P/L = 1/360 for ghosts and liposomes), the probe inserts itself at initially dilute sites to form a class of low-affinity sites consisting of clusters of variable size. At still higher P/L ranges (greater than 1/100), mu..

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University of Melbourne Researchers