Journal article

THERMOTROPIC LIPID PHASE-SEPARATION IN THE HUMAN IMMUNODEFICIENCY VIRUS

LM GORDON, FC JENSEN, CC CURTAIN, PW MOBLEY, RC ALOIA

BIOCHIMICA ET BIOPHYSICA ACTA | ELSEVIER SCIENCE BV | Published : 1988

Abstract

The presence of thermodependent lipid domains in the envelope of the human immunodeficiency virus (HIV) was studied. HIV was propagated in Hut-78 cells and purified by differential-gradient centrifugation. Since the virus was highly infectious in cell culture and Western blots of detergent-inactivated HIV showed envelope proteins when exposed to sera containing anti-HIV antibodies, this viral preparation was not deficient in 'spike' or 'knob' particles. Electron spin resonance (ESR) studies of intact HIV labeled with 5-nitroxide stearate (5-NS) indicated that a temperature-dependent lipid phase separation occurs with a high onset at approx. 42 degrees C and a low onset at approx. 15 degrees ..

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University of Melbourne Researchers