Journal article

TRIIODOTHYRONINE REGULATION OF MULTIPLE RAT HEPATIC GENES - REQUIREMENT FOR ONGOING PROTEIN-SYNTHESIS

PS HAMBLIN, A SANTOS, NCW WONG, HL SCHWARTZ, JH OPPENHEIMER

MOLECULAR ENDOCRINOLOGY | ENDOCRINE SOC | Published : 1987

Abstract

We have examined the role of rapidly turning over proteins in the T3 regulation of multiple rat hepatic genes. T3 induction of the rapidly responsive mRNA-S14 was markedly inhibited by cycloheximide (1 mg/100 g BW) or emetine (3 mg/100 g) injected ip 30 min before T3 (mRNA-S14 concentration was only 35% of that in T3-treated controls 8.5 h after administration of either protein synthesis inhibitor, P less than 0.01). Cycloheximide exhibited a similar effect on each of five other more slowly responsive T3 regulated genes. When cycloheximide was given 10 h after T3, the expected T3-induced rise of mRNA-S7 activity was completely prevented, and for mRNA-S4 activity the anticipated rise was blun..

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University of Melbourne Researchers