Journal article
Human CD1c (BDCA-1) myeloid dendritic cells secrete IL-10 and display an immuno-regulatory phenotype and function in response to Escherichia coli
AJ Kassianos, MY Hardy, X Ju, D Vijayan, Y Ding, AJE Vulink, KJ Mcdonald, SL Jongbloed, RB Wadley, C Wells, DNJ Hart, KJ Radford
European Journal of Immunology | WILEY-BLACKWELL | Published : 2012
Abstract
Human blood myeloid DCs can be subdivided into CD1c (BDCA-1) + and CD141 (BDCA-3) + subsets that display unique gene expression profiles, suggesting specialized functions. CD1c + DCs express TLR4 while CD141 + DCs do not, thus predicting that these two subsets have differential capacities to respond to Escherichia coli. We isolated highly purified CD1c + and CD141 + DCs and compared them to in vitro generated monocyte-derived DCs (MoDCs) following stimulation with whole E. coli. As expected, MoDCs produced high levels of the proinflammatory cytokines TNF, IL-6, and IL-12, were potent inducers of Th1 responses, and processed E. coli-derived Ag. In contrast, CD1c + DCs produced only low levels..
View full abstractGrants
Awarded by National Health and Medical Research Council of Australia
Funding Acknowledgements
This study was supported by projects grants 382308 (D.N.J.H. and K.J.R.) and 604306 (K.J.R.) from the National Health and Medical Research Council of Australia, and the Cancer Council Queensland (K.J.R.). A.J.K. was supported by an NHMRC Dora Lush Postgraduate Research Scholarship and M.Y.H. was supported by a University of Queensland Postgraduate Research Scholarship. K.J.R. is supported by an NHMRC CDA Level 2 Fellowship. We thank Mr. Michael Lobb and Associate Professor Ross Norris (Australian Centre for Paediatric Pharmacokinetics) for the HPLC studies, volunteer donors, and Stephanie Diaz-Guilas and Sonia Hancock (MMRI) for the collection of leukapheresis samples.