Journal article

Transcriptomic analysis of wheat near-isogenic lines identifies PM19-A1 and A2 as candidates for a major dormancy QTL

Jose M Barrero, Colin Cavanagh, Klara L Verbyla, Josquin FG Tibbits, Arunas P Verbyla, B Emma Huang, Garry M Rosewarne, Stuart Stephen, Penghao Wang, Alex Whan, Philippe Rigault, Matthew J Hayden, Frank Gubler

Genome Biology | BMC | Published : 2015

Abstract

BACKGROUND: Next-generation sequencing technologies provide new opportunities to identify the genetic components responsible for trait variation. However, in species with large polyploid genomes, such as bread wheat, the ability to rapidly identify genes underlying quantitative trait loci (QTL) remains non-trivial. To overcome this, we introduce a novel pipeline that analyses, by RNA-sequencing, multiple near-isogenic lines segregating for a targeted QTL. RESULTS: We use this approach to characterize a major and widely utilized seed dormancy QTL located on chromosome 4AL. It exploits the power and mapping resolution afforded by large multi-parent mapping populations, whilst reducing complexi..

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Grants

Funding Acknowledgements

We thank Kerrie Ramm, Trijntje Hughes and Jasmine Rajamony for their excellent technical assistance and the CSIRO MAGIC team for their dedication and help in the field and during the dormancy screenings. We also thank Dr. Jen Taylor and Dr. Jean Finnegan for their suggestions to improve the manuscript, and Dr. Scott Boden, Dr. Maria M. Alonso-Peral and Prof. Daryl Mares for their critical reviews. This work was supported by the Grains and Research Development Corporation and by the CSIRO.