Recognition of the major cell surface glycoconjugates of Leishmania parasites by the human serum mannan-binding protein.
PJ Green, T Feizi, MS Stoll, S Thiel, A Prescott, MJ McConville
Mol Biochem Parasitol | Published : 1994
Activation of complement on the surface of parasitic protozoa of the genus Leishmania appears to be important for parasite infectivity in the mammalian host, as it allows these parasites to attach to and invade macrophages via their surface complement receptors. Serum mannan-binding protein (MBP) is a known activator of complement. Therefore, in the present study, we have investigated whether serum MBP binds to live Leishmania parasites, and to mannose-containing saccharides derived from the parasite cell surface. We have observed by fluorescence microscopy that biotinylated MBP binds to the surface of L. major and L. mexicana promastigotes. At this developmental stage the parasites are coat..View full abstract