Plasmodium falciparum: The calmodulin gene is not amplified or overexpressed in chloroquine resistant or sensitive isolates

Abstract

The in vitro growth of Plasmodium falciparum is sensitive to some calmodulin antagonists and these compounds show antagonism with classic antimalarials such as chloroquine suggesting competition for the same drug binding site. In order to ask if calmodulin is involved in resistance to chloroquine we cloned the calmodulin gene of P. falciparum. We show that it is encoded by a single gene and that the putative protein is highly homologous to calmodulin from other eukaryotes. The calmodulin gene is encoded on chromosome 14 and contains a single intron of 506 bp that has the appropriate donor and acceptor splice sites. Two major transcripts of similar size are encoded by this gene. The sequence ..