Journal article

DNA POLYMORPHISMS AND SUBPOPULATIONS IN BABESIA-BOVIS

AF COWMAN, P TIMMS, DJ KEMP

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | ELSEVIER SCIENCE BV | Published : 1984

Abstract

Independent isolates of Babesia bovis differ by only a limited number of polypeptides, some of which may be important as host protective antigens. Avirulent derivatives of these parasites also differ from their virulent counterparts in only a few polypeptides. To identify genes encoding such polypeptides we have isolated cDNA clones corresponding to poly(A)+ RNAs that are expressed only in certain isolates. For this purpose a cDNA clone library was constructed from poly(A)+ RNA of the K-avirulent isolate (KA). These clones were screened by colony hybridization using [32P]cDNA complementary to poly(A)+ RNA from KA and from virulent isolates, in order to identify clones that selectively hybrid..

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University of Melbourne Researchers