Journal article
Molecular characterization and phylogenetic inferences of Dermanyssus gallinae isolates in Italy within an European framework
M Marangi, C Cantacessi, OAE Sparagano, A Camarda, A Giangaspero
Medical and Veterinary Entomology | WILEY | Published : 2014
DOI: 10.1111/mve.12050
Abstract
In order to investigate the genetic relationships between Dermanyssus gallinae (Metastigmata: Dermanyssidae) (de Geer) isolates from poultry farms in Italy and other European countries, phylogenetic analysis was performed using a portion of the cytochrome c oxidase subunit 1 (cox1) gene of the mitochondrial DNA and the internal transcribed spacers (ITS1+5.8S+ITS2) of the ribosomal DNA. A total of 360 cox1 sequences and 360 ITS+ sequences were obtained from mites collected on 24 different poultry farms in 10 different regions of Northern and Southern Italy. Phylogenetic analysis of the cox1 sequences resulted in the clustering of two groups (A and B), whereas phylogenetic analysis of the ITS+..
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Funding Acknowledgements
This work was supported by a research grant (Ricerca Corrente 2008-2010-Istituto Zooprofilattico Sperimentale della Puglia e Basilicata) from the Ministry of Health, Italy. C. C. is the grateful recipient of a Peter Doherty Early-Career Fellowship granted by the National Health & Medical Research Council (NHMRC) of Australia. The funders have no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.