RNA-Seq Reveals Infection-Induced Gene Expression Changes in the Snail Intermediate Host of the Carcinogenic Liver Fluke, Opisthorchis viverrini
Sattrachai Prasopdee, Javier Sotillo, Smarn Tesana, Thewarach Laha, Jutharat Kulsantiwong, Matthew J Nolan, Alex Loukas, Cinzia Cantacessi
PLOS NEGLECTED TROPICAL DISEASES | PUBLIC LIBRARY SCIENCE | Published : 2014
BACKGROUND: Bithynia siamensis goniomphalos is the snail intermediate host of the liver fluke, Opisthorchis viverrini, the leading cause of cholangiocarcinoma (CCA) in the Greater Mekong sub-region of Thailand. Despite the severe public health impact of Opisthorchis-induced CCA, knowledge of the molecular interactions occurring between the parasite and its snail intermediate host is scant. The examination of differences in gene expression profiling between uninfected and O. viverrini-infected B. siamensis goniomphalos could provide clues on fundamental pathways involved in the regulation of snail-parasite interplay. METHODOLOGY/PRINCIPAL FINDINGS: Using high-throughput (Illumina) sequencing ..View full abstract
Awarded by Thailand Research Fund through the Royal Golden Jubilee Ph.D. Program
Funding from the National Health & Medical Research Council (NHMRC) of Australia is gratefully acknowledged. AL and CC are supported by a principal research fellowship and an early-career research fellowship, respectively, awarded by the NHMRC. This work was also supported by Thailand Research Fund through the Royal Golden Jubilee Ph.D. Program (Grant No PHD/0027/2551) to SP and ST, and National Research University Project of Thailand, through the Health Cluster (SHeP-GMS), Office of the Higher Education Commission, Khon Kaen University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.