Identification of genes differentially regulated by the P210 BCR/ABL1 fusion oncogene using cDNA microarrays.
Petra Håkansson, David Segal, Carin Lassen, Urban Gullberg, Herbert C Morse, Thoas Fioretos, Paul S Meltzer
Exp Hematol | Published : 2004
OBJECTIVE: The t(9;22) translocation is associated with more than 95% of cases of chronic myeloid leukemia. The resulting fusion of the BCR and ABL1 loci produces the constitutively active BCR/ABL1 tyrosine kinase. A wide range of signal transduction molecules are activated by BCR/ABL1, including MYC, PI-3 kinase, and different STAT molecules. In contrast, relatively few genes are known to be regulated by BCR/ABL1 at the level of transcription. MATERIALS AND METHODS: In an effort to better understand the transcriptional program activated by BCR/ABL1, we used cDNA microarrays to evaluate the relative expression of approximately 6450 human genes in U937 myelomonocytic cells expressing P210 BCR..View full abstract