Journal article

A NOVEL KERATAN SULFATE PROTEOGLYCAN FROM A HUMAN EMBRYONAL CARCINOMA CELL-LINE

S COOPER, MF PERA, W BENNETT, JT FINCH

BIOCHEMICAL JOURNAL | PORTLAND PRESS LTD | Published : 1992

Abstract

We describe here the purification and partial characterization of a 200 kDa keratan sulphate proteoglycan found in the pericellular matrix of human embryonal carcinoma cells. Previously we have shown that this molecule is recognized by a monoclonal antibody (GCTM-2). The antigen was isolated using ion-exchange chromatography and gel filtration, purification being monitored by e.l.i.s.a. using GCTM-2. Metabolic labelling of GCT 27 C-4 embryonal carcinoma cells with sodium [35S]sulphate resulted in the incorporation of [35S]sulphate into the purified molecule. Throughout the purification procedure, the peaks of 35S radioactivity were coincident with the peaks of immunoreactivity, and this labe..

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University of Melbourne Researchers